Principles of healing of virus-infected raspberry varieties by chemotherapy in vitro

  • T. Medvedeva
  • T. Natalchuk
  • K. Suprun
  • I. Ryaba
  • N. Triapitsyna
Keywords: virazole, raspberry explants, ideovirus, semi-quantitative LIA, nepoviruses, chemotherapy


Most of promising raspberry varieties need to be heal from complex viral infections. Development of the most standardized and universal plant healing methods — one of the actual tasks of biotechnology works for raspberries plant material production. The decrease of virus concentration in explants of some promising hybrid forms and varieties of raspberry affected by complex viral infection were analyzed with semi quantitative LIA method after explants treatment with ribavirin. Rated the therapeutic, toxic and prolonged effect of ribavirin for elimination of four viruses were evaluated. It was revealed universal peculiarities for chemotherapy of raspberries shoots using this virocide.


Pữpola N., Lepse L., Kặle A. Occurrence of RBDV in Latvia and virus elimination in vitro by chemotherapy. Scientific works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture, Sodininkystė ir Daržinikystė. 2009. №28(3). P. 165—172.

Wang Q.-C., Valkonen J.P.T. Cryotherapy of shoot tips: novel pathogen eradication method. Trends in Plant Science. 2009. №14 (3). P. 119—122.

Wang Q.-C. Cuellar W. J., Rajamäki M.-L. Combined thermotherapy and cryotherapy for efficient virus eradication: relation on virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology. 2008. №9 (2). P. 237—250.

Polák J. Oukropec I. Identification of interspecific Peach and Prunus sp. Hybrids resistant to Plum pox virus infection. Plant Protect. Sci. 2010. №46(4). Р. 139—144.

Chen D.M., Zhang S.L., Jin Y.F. A method for genomic DNA preparation of woody fruit crops. J. Zhejiang Agri. Uni. 1997. № 23. Р. 261—264.

Kalendar R, Antonius K., Smykal P. et al. PBS: a universal method for DNA fingerprinting and retrotransposon isolation. Theor Appl Genet. 2010. № 121. Р. 1419—1430.

Antonius-Klemola K., Kalendar R., Schulman A.H. TRIM retrotransposons occur in apple and are polymorphic between varieties but not sports. Theoretical and Applied Genetics. 2006. №112 (6). Р. 999—1008.

How to Cite
Medvedeva, T., Natalchuk, T., Suprun, K., Ryaba, I., & Triapitsyna, N. (2018). Principles of healing of virus-infected raspberry varieties by chemotherapy in vitro. Interdepartmental Thematic Scientific Collection of Plant Protection and Quarantine, (64), 90-98.