Reproduction of entomopathogenic nematodes of the genera Steinernema Travassos, 1927 (Rhabditida: Steienernematidae) within strawberry blossom weevil larvae Anthonomus rubi Herbst, 1795 (Coleoptera: Curculionidae)

  • D. Sigareva
  • V. Kharchenko
Keywords: entomopathogenic nematodes, genus Steinernema, laboratory propagation of EPN, raspberry-strawberry weevil Anthonomus rubi

Abstract

Studies have been conducted on the contamination in the laboratory of larvae of raspberry-straw weevil (Anthonomus rubi Herbst, 1795) with two isolates of entomopathogenic nematodes (ETNs) of the genus Steinernema Travassos, 1927, vchecked the possibility of their reproduction under these conditions, and features of quantitative performance indicators. On the basis of the obtained data, it was established that, regardless of the type of isolate of the EPN, all experimental A. rubi larvae died on the 3rd day after infection, and the yield of larvae nematodes from the dead insects began on day 10—12 and lasted 42—44 days. During this period, on average, from one of the affected specimens of raspberry-straw weevil, from 26965 to 29168 larvae of the EPN was allocated. The total number of larvae of EPN obtained in the infection of 14 larvae of weevil of raspberry-strawberry was 392929 individuals. The migration of newly-formed individuals of the EPN was uneven, the bulk of the larvae (61—66.2%) is released during the second decade of the month, the rest — 24.5%—31.2% in the third decade. In the first and last decades the larvae are significantly less (3.5—5.8%).

References

Sigareva D.D., Nikishicheva K.S. (2005). Biologicheskie preparaty na osnove jetomopatogennyh nematod kak jekologicheski bezopasnyj sposob regulirovanija chislennosti nasekomyh. [Biological preparations based on etiopathogenic nematodes as an ecologically safe way to regulate the number of insects]. Vestnik zoologii. Otdel'nyj vypusk № 19: Parazitologija i sovremennost': materialy nauchno-prakticheskoj konferencii Ukrainskogo nauchnogo obshhestva parazitologov, posvjashhennoj 100-letiju so dnja rozhdenija akademika NAN Ukrainy A.P. Markevicha. Ch. 2. S. 298—301. [in Russian].

Danilov L.G., Ajrapetjan V.G., Nashhekina T.Ju. (2010). Biologicheskie preparaty na osnove jentomopatogennyh nematod. [Biological preparations based on entomopathogenic nematodes]. Zashhita i karantin rastenij. №2. S. 32—33. [in Russian].

Siharova D.D., Halahan T.O., Dovhelia V.M. et al. (2012). Otsinka invaziinoi aktyvnosti entomopatohennykh nematod rr. Steinernema ta Heterorhabditis vidnosno komakh. [Evaluation of invasive activity of entomopathogenic nematodes of Steinernema and Heterorhabditis against insects]. Ahrobiolohiia. № 8. S. 140—145. [in Ukrainian].

Nickle W.R. (1991). Manual of agricultural nematology. New York: Marcel Dekker Inc. 1035 pp.

Published
2018-11-19
How to Cite
Sigareva, D., & Kharchenko, V. (2018). Reproduction of entomopathogenic nematodes of the genera Steinernema Travassos, 1927 (Rhabditida: Steienernematidae) within strawberry blossom weevil larvae Anthonomus rubi Herbst, 1795 (Coleoptera: Curculionidae) . Interdepartmental Thematic Scientific Collection of Plant Protection and Quarantine, (64), 184-190. https://doi.org/10.36495/1606-9773.2018.64.184-190