Виявлення нових осередків поширення карантинних органiзмів — ґрунтових збудників хвороб картоплі

A. Zelya; R. Suhareva; G. Zelya; M. Nikoriuk; O.  Kuvshynov

doi:10.36495/1606-9773.2020.66.82-93

A. Zelya Ukrainian science-research plant quarantine station of the Institute of Plant Protection of NAAS, 1, Naukova str, v. Boiany, Novoselitsa district, Chernivtsi region, 60321, Ukraine
R. Suhareva Ukrainian science-research plant quarantine station of the Institute of Plant Protection of NAAS, 1, Naukova str, v. Boiany, Novoselitsa district, Chernivtsi region, 60321, Ukraine
G. Zelya Ukrainian science-research plant quarantine station of the Institute of Plant Protection of NAAS, 1, Naukova str, v. Boiany, Novoselitsa district, Chernivtsi region, 60321, Ukraine
M. Nikoriuk Ukrainian science-research plant quarantine station of the Institute of Plant Protection of NAAS, 1, Naukova str, v. Boiany, Novoselitsa district, Chernivtsi region, 60321, Ukraine
O. Kuvshynov Ukrainian science-research plant quarantine station of the Institute of Plant Protection of NAAS, 1, Naukova str, v. Boiany, Novoselitsa district, Chernivtsi region, 60321, Ukraine

DOI: https://doi.org/10.36495/1606-9773.2020.66.82-93

Keywords: quarantine organisms; identification; potato cancer; zoosporangia; potato nematodes; cysts; foci of spread; infectious load

Abstract

Goal. To determine potato’s quarantine pests causative agents of potato wart and nematodes with determining degree of soil’s infectious load by disease causative agents.

Methods. Materials for researches were soils’ samples from different disease sources. The soil’s samples selection from one soil’s probe was conducted as per standard method ДСТУ (DSTU) 3355-96. Points of soil’s samples was fixed by GPS — navigator Garmin eTrex Legend. The potato wart causative agent determination and cysts of yellow potato cyst nematodes was conducted by flotation method in device «RUTA» in sodium iodide solution. The zoosporangia viability determining conducted with solution Coomassi blue G-250 usage.

Results of conducted researches for determining potato pests causative agents with potato wart pathogens in Carpathian region of Ukraine in 2019—2020 presented. The study showed different disease level of soil’s infecting by zoosporangia and potato cysts nematodes. The load was consisted of 56—68 zoosporangia/1 g of soil, 14—18 cysts of yellow potato cyst nematode, relatively. There were determined 42—48 zoosporangia/1g soil in Zakarpattia region, 40—45 zoosporangia in Ivano-Frankivsk region, 49 in Turka, Lviv region and 0 nematodes, relatively.

Conclusions. Apparatus «RUTA» is designed for potato quarantine pests determining. It is allowed to determine cysts of potato yellow cysts and causative agent of potato wart zoosporangia. The potato wart analysis and yellow potato cyst nematode infectious load Different infectious level of soil’s present disease infecting analysis showed.

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Detection of new foci of distribution of quarantine organisms — soil causative agents of potato diseases

Abstract

References