Formation of biocomplexes based on the bacteria Pseudomonas fluorescens and substances of a stimulating nature to limit the development of harmful organisms in potatoes
Abstract
Goal. Selection of combinations of biocomplexes based on Pseudomonas fluorenscens bacteria with stimulant preparations based on various derivatives of ammonium salts of dihydropyrimidine and study of their effectiveness.
Methods. Biotechnological methods for the study of bacteria Pseudomonas fluorenscens strain AR-33. The concentration of viable bacteria (CFU/cm3) was determined by the Koch method. Accounts were performed according to generally accepted methods using experimental methods in phytopathology and plant protection. Determined the effectiveness of drugs at different rates of consumption against fungal diseases.
Results. Derivatives of ammonium salts of dihydropyrimidine did not show a toxic effect on reducing the concentration of viable cells of strain AR-33 bacteria Pseudomonas fluorescens. The best indicators of the weight of 100 seeds and the number of beans in soybean plants showed a combination: Planriz, v.s. (bacteria of strain AR-33 Pseudomonas fluorescens, 3 ќ 10 9 CFU/cm3) (5 l/ha) + 0.1% solution of ximedon + 0.2% solution of succinic acid + 2 ml of DMAE + 2 ml of DMSO. The use of all combinations of biocomplexes showed the effectiveness of drugs against diseases in the range of 59.31—69.63%. With the use of biocomplexes, due to the fungicidal, immunoprotective and stimulating effect, a yield increase of 1.15—1.7 times relative to the control was recorded. The best yield on potatoes (3.4 t/ ha) was provided by the combination Planriz, v.s. (5 l/ha) + 0.1% solution of ximedon + 0.2% solution of succinic acid + 2 ml of DMAE + 2 ml of DMSO. The effectiveness of the drug against late blight was 79.1%.
Conclusions. The use of stimulants and excipients DMAE and DMSO as substances that affect various transmembrane functions, provided an increase in the effectiveness of drugs by 8—14% relative to combinations without their use.
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