The role of growth regulators in the ontogenesis of plants of potato cancer differentiators in vitro culture

Abstract

Goal. To study impact of growing regulators on morphometric indexes and potato cultivar-differentiators productivity in culture in vitro.

Methods. The studies conducted in the Laboratory of Biotechnology for Agricultural Crops of Ukrainian Plant Quarantine Research Station Institute of Plant Protection of NAAS. Murashige-Skoog (MS) nutrient medium was used with the addition of different concentrations of Epin-maxi growth regulators (MS — control; MS + Epin-maxi r., (2,4-epibrassinolide, 0.012 g/l); MS + Epin-maxi, 0.025 g/l) and Poteitin (MS — control; MS + Poteitin w.s., (2,6-dimethylpyridine-1-oxide with succinic acid, 0.15 mg/l); MS + Poteitin, 0.3 mg/l) on different varieties of potato cancer of different ripeness groups (Schedryk — early, Slovyanka — medium-ripe). Plants grew in terms of cultivation room during 16-hour photoperiod a light intensity of 2000—2500 lux, temperature 22—25°C and humidity of 60—80%. The grafting conducted in quantity 25 plants with three times experiment repeating.

Results. The morphogenesis optimal indexes received during their put into the nutritious medium MS + Epin-maxi 0.025 g/l and MS + Poteytin 0.3 mg/l. The plants’ height of variety Schedryk increased on 21^st day of growing on 4.9—25.2 mm, in control (65.2 mm) and internodes number increased 0.4—0.9 pcs in control (5.2 pcs.). The plant’s height increased of variety Slovyanka in variants with stimulators was more than on 12.1—28.4 mm and the internodes number increased 0.5—1.1 pcs. The root-forming increase determined in all experiments variants: roots quantity — on 2.6—3.4 pcs., their length was 25.6—29.8 mm, the average microtubers weight was in 1.4—1.6 times higher, the mictotubers weight increase on 1 plant in 1.6—1.9 times, the quantity of microtubers on one plants in 1.6—1.8 times in comparison with control.

Conclusions. The optimal indexes of morphometric parameters and potato cultivar-differentiators productivity on culture in vitro received in variants of research with Epin-maxi 0.025 g/l and Poteytin 0.3 mg/l. These stimulators, when added to the nutrient medium, contributed to an increase in plant height, the number of internodes, root formation (number of roots and their length), average mass of microtubers, mass and number of microtubers per plant.