Screening of collection strains to create association of microorganisms with nematocidal activityscreening

N. Pyliak; L. Loban

doi:10.36495/PHSS.2024.70.246-256

N. Pyliak Engineering and Technological Institute «Biotechnika» of the NAAS, 26, Mayatska road, Khlibodarske township, Odesa district, Odesa region, 67667, Ukrain https://orcid.org/0000-0002-5074-4011
L. Loban Engineering and Technological Institute «Biotechnika» of the NAAS, 26, Mayatska road, Khlibodarske township, Odesa district, Odesa region, 67667, Ukrain https://orcid.org/0009-0001-4437-4480

DOI: https://doi.org/10.36495/PHSS.2024.70.246-256

Keywords: dalls nematodes, collection microorganisms, complex biopreparations, microscopic fungi, nematocidal activity, cyst-forming nematodes

Abstract

Goal. Selection of collection strains to create a stable association of microorganisms, which will serve as the basis of a new complex drug against gall and cyst-forming nematodes.

Methods. A complex of generally accepted methods and methodical approaches was applied for the screening of collection strains with nematophagous efficiency.

Results. Eight collection microorganisms were screened in laboratory conditions. Five strains of fungal etiology with nematicidal activity against cyst-forming and gall nematodes were selected for further research. The relationships, viability, dynamics of growth and development of potential biological control agents of phytoparasitic nematodes on agarized nutrient media were studied.

Conclusions. During joint cultivation of the investigated microorganisms on agarized nutrient media, it was established that Orbilia oligospora, Hirsutella rhossiliensis, Metаrhizium anisopliae, Purpureocillium lilacinus, Metacordyceps chlamydosporia strains do not exhibit antagonistic properties and can be used to create a stable association of microorganisms with nematocidal activity.

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