Hormonal preparations on the productivity and morphological parameters of potato wart differentiator varieties indicators in culture in vitro

T. Safronova

doi:10.36495/PHSS.2024.70.267-276

T. Safronova Ukrainian Research Plant Quarantine Station of Institute of Plant Protection of the NAAS, 4, Naukova str., p. Boyany, Chernivtsi district, Chernivtsi region, 60321, Ukraine

DOI: https://doi.org/10.36495/PHSS.2024.70.267-276

Keywords: potato wart, cultivars-differentiators, culture in vitro, phytohormones, growth regulators, microclonal reproduction

Abstract

Goal. To study the impact of hormonal origin preparations under controlled conditions on the ontogenesis and productivity of potato varieties-differentiators in culture in vitro.

Methods. The researches conducted in biotechnological laboratory of agriculturaal crops Ukrainian science-research plant quarantine station Institute of Plant Protection NAAS. The Murasige-Skoog (MS) nutrient medium was used with adding of various concentration of growth regulators: Epin-Maxi (2.4-epibrassinolide, 0.012 g/l) and Poteytin (2.6-dimethylpyridine-1-oxide with succinic acid, 0.15 mg/l) in the presence of adenine (0.5 mg/l) in the medium during the experiment. The study was conducted on potato wart cultivar — differentiators of different ripeness groups: Glazurna — early, Chervona Ruta-medium-ripened. The plants were grown in a cultivation room at a 16-hour photoperiod with a lighting intensity of 2000—2500 Lx, a temperature of 21—25°C and an air humidity of 60—80%. Each cultivar cuttings were carried out in the amount of 25 plants, with three times repetition of the experiment.

Results. The morphogenesis were the highest in comparison with control during the input into the nutrient medium MS growth medium Epin-Maxxi (0.025 g/l) or Poteytin (0.3 mg/l). The increased ability to grow and form more internodes was recorded on 10—15% on 21 st day of cultivation. The root forming intensity was determined during the present phytohormones adding. The microtubers average number and average mass increased.

Conclusions. The researched preparations usage allowed to increase productivity cultivar-differentiators for wart potato in culture in vitro with minimal costs. Growth stimulators Epin-Maxi and Poteytin increased the morphometric indexes at plants during their input into the medium. They favored the culture growth and development. They favored the root-forming of potato plants and microtubers quantity increase. Adenine, is as a component of nucleic acids and coenzymes. It plays an important role in plant metabolism. It favors the effective energy usage and impacts on photosynthesis intensity. It causes the Adenine impact as cytoconin on potato growth, development and resistance to stress factors (drought, excessive moisture nutrient lack).

References

European and Mediterranean plant protection organization. (2014). Eradication of Synchytrium endobioticum from Austria. European and Mediterranean Plant Protection Organization (EPPO). May 1. Last accessed June 26, 2014. URL: http://archives.eppo.int/EPPOReporting/2014/Rse-1405.pdf

Ballvora A., Flath K., Lübeck J., Strahwald J., Tacke E., Hofferbert H.-R., Gebhardt C. (2011). Multiple alleles for resistance and susceptibility modulate the defense response in the interaction of tetraploid potato (Solanum tuberosum) with Synchytrium endobioticum pathotypes 1, 2, 6 and 18. Theoretical and Applied Genetics, 123(8), 1281-1292.

European and Mediterranean plant protection organization. (2017). Bulletin OEPP/EPPO Bulletin. National regulatory control systems PM 9/5 (2) Synchytrium endobioticum, 47(3), 511-512.

Hampson M.C. (1996). A qualitative assessment of wind dispersal of resting spores of Synchytrium endobioticum, the causal agent of wart disease. Plant Disease, (80), 779-782.

European and Mediterranean plant protection organization. (2004). EPPO Standards PM 7/28. Diagnostic protocols for regulated pests: Synchytrium endobioticum. Bull. OEPP/EPPO Bull., (34), 213-218.

Zelya A., Zelya G., Oliynyk T., Pylypenko L., Solomiyciuk M., Kordulean R., Skoreyko A., Bunduc Yu., Ghunchak V. (2018). Screening of potato varieties for multiple resistance to Synchytrium endobioticum in the western region of Ukraine. Agricultural science and practice, 5(3), 3-131. Doi: http://doi.org/10.15407/agrisp5.03.003

Oliinyk T.M., Bondarchuk A.A., Slobodian S.O. et all. (2013). Ozdorovlennia sortiv kartopli metodom kultury apikalnykh merystem u poiednanni iz khimioterapiieiu. Metodychni rekomendatsii. [Potato varieties treating by culture method of apical meristems with chemical therapy]. Instytut kartopliarstva NAAN Ukrainy. Nemishaieve, P. 52. (in Ukrainian).

Skoreiko A.M., Andriichuk T.O., Bilyk R.M., Safronova T.V. (2021). Optymizatsiia klonalnoho mikrorozmnozhennia sortiv-dyferentsiatoriv raku kartopli. [Optimization of microclonal reproduction of potato wart cultivar-differentiators]. Zakhyst i karantyn roslyn, 67, 242-250. Doi: http://doi.org/10.36495/1606-9773.2021.67.242-250 (in Ukrainian).

Mohapatra P.P., Batra V.K. (2017). Tissue culture of potato (Solanum tubero-sum L.): a review. International Journal of Current Microbiology and Applied Sciences, 6(4), 489-495. Doi: http://doi.org/10.20546/ijcmas.2017.604.058

Skoreiko A.M., Andriichuk T.O., Bilyk R.M., Safronova T.V. (2022). Rol rehuliatoriv rostu v ontohenezi roslyn sortiv-dyferentsiatoriv raku kartopli v kulturi in vitro. [The role of growth regulators in the ontogenesis of plants of potato cancer differentiators in vitro culture]. Fitosanitarna bezpeka, 68, 148-155. Doi: http://doi.org/10.36495/1606-9773.2022.68.148.155 (in Ukrainian).

Tsilyurik O.I., Izhboldin O.O., Sologub I.M. (2022). Vplyv stymuljatoriv rostu na biometrychni pokaznyky ta urozainist kukurudzy v Pivnichnomy Stepu. [Effect of growth stimulants on biometric indicators and yield of corn in the Northern Steppe]. Agrarian innovations, (15), 59-66. Doi: https://doi.org/10.32848/agrar.innov.2022.15.9 (in Ukrainian).