Morphological-cultural and physiological-biochemical features of isolates of pathogenic bacteria of the Agaricus bisporus
Abstract
Purpose. The study of the functional features of pathogenic bacteria, which we are isolated from Agaricus bisporus.
Methods We used biotechnological research methods. We determined the trophic features of pathogenic bacteria using biochemical methods. This is the development of biotechnology to control their distribution. We determined the oxidase activity of bacteria according to the Kovach method (on a membrane filter that we previously wetted with NN-dimethyl-p-phenylene-diamine sulfate. We determined the catalase activity by adding 10% hydrogen peroxide solution to the culture drop.
Results. We determined the sources of carbon nutrition pathogenic bacteria using a test system Isolate 9.4 utilizes sugars such as xylose, dextrose, galactose, which are monosaccharides and melibiosis, L-arabinose, mannose, ONPG (ortho-nitrophenyl-β-galactopyranose), esculin, citrate, m Alonate. Isolate 6.2 showed a positive result for xylose, dextrose during testing. Isolate 6.1 uses the following sugars: dextrose, trehalose, melibiosis, mannose. Isolate 9.5 indicates the presence of ONPG, esculin, citrate and malonate and the absence of catalase. Isolates 11.1 and 9.5 have a positive reaction to oxidase. Isolates 6.2 and 13.2 had a weak reaction. Sources of carbon nutrition revealed sugars of the monosaccharide group (xylose, dextrose, galactose), polysaccharides and amino acids. The reaction to the breakdown of carbohydrates showed that all isolates had an oxidative type of metabolism.
Conclusions. We investigated the morphological and cultural features of isolates of pathogenic bacteria of champignon bicuspid isolated from Agaricus bisporus. We studied the physiological and biochemical properties of bacteria and reactions to the breakdown of carbohydrates in a synthetic medium, the oxidase activity of bacteria according to the Kovacs method, and catalase activity. We carried out the identification of the obtained isolates, comparing them with the properties of bacteria of the causative agents of bacteriosis of this fungus, already described in the articles and in the Bergey Bacteria Guide.
References
Kalac P. A. (2013). Review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. Journal of the Science of Food and Agriculture;93(2): P.209–218. doi: 10.1002/jsfa.5960 [in English].
Ivanova T.V. (2018). Biotechnologiya hrybiv. [Biotechnology of mushrooms]. V.2. Komprynt. 165 p. [in Ukrainian].
Sanguinetti M. (2015). Edible Mushrooms: Improving Human Health and Promoting Quality Life. International Journal of Microbiology. 2015: 376387: doi: 10.1155/2015/376387. [in English].
Pereima, I. V., Ivanova, T. V. (2017). Stimulation of growth of species of the fungus of the genus Pleurotus (Fr.) P. Kumm. at a glucose nutrition. Biotechnologia Acta., 10(6), P. 45–52. doi: org/10. 15407 / biotech10.06.045. [in English].
Patyka V.P., Pasichnyk L.A., Dankevych L.A. (2014). Diagnostyka phytopatogennyh bakterii: Metodychni rekomendatsii. [Diagnostic of phytopathogenic bacteria: Guidelines]. Kyiv. 57 p. [in Ukrainian].
Ivanova T.V., Kovalyshyna H.M. (2018). Biotekhnolohiyi Otrymannya mitseliyu Pleurotus ostreatus na zerni riznykh sortiv pshenytsi. [Biotechnologies for obtaining Pleurotus ostreatus mycelium on grains of different wheat varieties]. Zbir. nauk. pratsʹ Myronivsʹkyy visnyk. T. 6. Р. 25–30. [in Ukrainian].
Ivanova, T.V. et all. (2015). New approaches extraction of viral RNA from edible mushrooms. Scientific Journal «ScienceRise». 1 (15). P. 44—46.
Radchenko О. S. (2012). Fisiologo-biokhimichni vlastyvosti mikroorganizmiv ta metody yih vyznachennya: Navchalniy posibnyk. [Physiological and biochemical properties of microorganisms and methods their determination: Tutorial]. Agrar media group. 211 p. [in Ukrainian].
Patyka V.P., Pasichnyk L.A., Gvozdyak R.І., Petrychenko V.F., Korniychuk О.V., Kalinichenko A.V., Butsenko L.M., Zhytkevych N.V. (2017). Phytopatogenni bakterii. Metody doslidzhen. [Phytopathogenic bacteria. Research methods]. Vindruk. 432 p. [in Ukrainian].
Gadzalo Ya. M., Patyka M.V., Zaryshnyak A.S. (2015). Agrobiologiya ryzosphery roslyn. [Agrobiology of the rhizosfere of plants]. Agrarna nauka. 368 p. [in Ukrainian].
Patyka M.V., Kolodyazhnii A.Yu., Ibatullin I.I. (2016). Otsinka metagenomu ta vyyavlennya funktsionalno znachuschyh polimorfizmiv prokariotiv gruntu metodom pirosektsionuvannya. [Metagenome evaluation and detection of functionally significant polymorphisms of soil prokaryotes by pyrosequencing]. Microbiologichnii journal, 78 (2). P. 43–51. [in Ukrainian].
Patyka M.V., Patyka V.P., Patyka T.I., (2009). Filohenetychni vzayemozv’yazky mizh serolohichnymy variantamy Bacillus thuringiensis. [Phylogenetic relationships between serological variants of Bacillus thuringiensis]. Biopolimers and Cell. 25 (3) : С. 240–244. http://dx.doi.org/10.7124/bc.0007E2 [in Ukrainian].
Orlova O.V., Vorobyova N.I., Svyrydova O.V., Andronov E.YE., Kolodyazhnyy A.YU., Moskalevsʹka YU.P., Patyka M.V. (2015). Sklad ta Funktsionuvannya mikrobnikh uhrupovanʹ pry rozkladanni solomy zlakiv u dernovo-pidzolystomu hrunti. [The composition and functioning of microbial communities in the decomposition of cereal straw in sod-podzolic soil]. Silʹsʹkohospodarsʹka biolohiya. 50 (3). P. 305–314.
Patyka M.V., Kruhlov YU.V., Berdnykov A.M., Patyka V.F. (2008). Rolʹ Linum usitatissimum L. u formuvanni mikrobnykh uhrupovanʹ pidzolystykh gruntiv. [The role of Linum usitatissimum L. in the formation of microbial groups of podzolic soils]. Mikrobiolohichnyy zhurnal. 70 (1). P. 59–70.
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