Polymorphism of Ukrainian common winter wheat cultivars with respect to the molecular marker for the gene conferring moderate resistance against Fusarium head blight

N. Kozub; O. Sozinova; A. Karelov; I. Sozinov; I. Kucheriavyi; V. Tishchenko; M. Batashova; O. Gusenkova; Ya. Blume

doi:10.36495/1606-9773.2019.65.87-99

N. Kozub Institute of Plant Protection of NAAS of Ukraine, Vasylkivska st., 33, Kyiv, Ukraine, 03022
O. Sozinova Institute of Plant Protection of NAAS of Ukraine, Vasylkivska st., 33, Kyiv, Ukraine, 03022
A. Karelov Institute of Plant Protection of NAAS of Ukraine, Vasylkivska st., 33, Kyiv, Ukraine, 03022
I. Sozinov Institute of Plant Protection of NAAS of Ukraine, Vasylkivska st., 33, Kyiv, Ukraine, 03022
I. Kucheriavyi Institute of Plant Protection of NAAS of Ukraine, Vasylkivska st., 33, Kyiv, Ukraine, 03022
V. Tishchenko Poltava State Agrarian Academy, Skovorody Str., 1/3, Poltava, 36003, Ukraine
M. Batashova Poltava State Agrarian Academy, Skovorody Str., 1/3, Poltava, 36003, Ukraine
O. Gusenkova Poltava State Agrarian Academy, Skovorody Str., 1/3, Poltava, 36003, Ukraine
Ya. Blume Institute of Food Biotechnology and Genomics, NAS of Ukraine, 2a, Osypovskogo Str., Kyiv, 04123, Ukraine

DOI: https://doi.org/10.36495/1606-9773.2019.65.87-99

Keywords: common wheat, disease resistance genes, Fusarium head blight, molecular markers

Abstract

Goal. The goal of the work was to evaluate the genetic potential of resistance to Fusarium head blight of collections of winter common wheat cultivars based on the allelic state of the TDF_076_2D gene conferring moderate resistance against Fusarium graminearum Schwabe and F. culmorum (W.G.Sm.) Sacc. fungi.

Materials and methods. We studied 68 winter common wheat cultivars developed in different scientific-breeding institutions of Ukraine (the Myronivka Remeslo Institute of Wheat of NAAS, the Plant Breeding and Genetics Institute (PBGI), the Рlant Production Institute nd. a. V. Ya. Yuryev of NAAS, Poltava State Agrarian Academy (PSAA)). A silica-based commercial kit was used for DNA extraction. To detect the allelic state of the resistance gene, the INDEL1 marker cosegregating with the TDF_076_2D gene was used. Amplified fragments obtained as a result of PCR were separated in 3% agarose gel and visualized with use of ethidium bromide.

Results. The frequency of the resistance allele according to the INDEL1 marker for the gene conferring moderate resistance to the Fusarium fungi ranged from 0.682 in the sample of PBGI cultivars to 0.882 in the PSAA group; the frequency of the resistance allele in the total sample made up 0.782. It was found that the cultivar ‘Poliovyk’ showed a non-typical pattern of amplified bands, which possibly indicates the allele for susceptibility to Fusarium head blight.

Conclusions. The majority of the common wheat cultivars from the studied sample carry the resistance allele of the marker for the gene of interest. The data obtained are consistent with the results of the previous research of the wider sample of the winter and spring common wheat cultivars. The cultivars with the resistance allele might show a lower infection level in the field and serve as a source of the gene in marker assisted selection. The cultivar ‘Poliovyk’ is worth more detailed studying with use of other molecular markers for the TDF_076_2D gene and (or) sequencing to find out a precise allelic state of the gene.

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